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Publications

Research at Iowa State University

  1.  Krishnamurthy, A.; Anand, R. K. Electrokinetic Desalting and Salting of Water-in-Oil Droplets. Anal. Chem. 2024. DOI: 10.1021/acs.analchem.4c00534Figure 1
  2. Kim, S.; Saurabh, K.; Khanwale, M. A.; Mani, A.; Anand, R. K.; Ganapathysubramanian, B. Direct Numerical Simulation of Electrokinetic Transport Phenomena in Fluids: Variational Multi-Scale Stabilization and Octree-Based Mesh Refinement. Journal of Computational Physics 2024. DOI: 10.1016/j.jcp.2023.112747.
  3. Rahn, K. L.; Osman, S. Y.; Pollak, Q. G.; and Anand, R. K. Electrokinetic Focusing of SARS-CoV-2 Spike Protein via Ion Concentration Polarization in a Paper-Based Lateral Flow Assay. Anal Methods 2023. DOI: 10.1039/d3ay00990d. Graphical abstract: Electrokinetic focusing of SARS-CoV-2 spike protein via ion concentration polarization in a paper-based lateral flow assay
  4. Kim, S.†;Krishnamurthy, A.†; Kasiviswanathan, P.; Ganapathysubramanian, B.; and Anand, R. K. In-droplet electromechanical cell lysis and enhanced enzymatic assay driven by ion concentration polarization. Anal. Chem. 2023. DOI: 10.1021/acs.analchem.3c02414.
  5. Osman, S.; Claus, E.L.; and Anand, R.K. Label-Free, Non-Optical Readout of Bead-Based Immunoassays with and without Electrokinetic Preconcentration. Anal. Chem. 2023. DOI: 10.1021/acs.analchem.3c01407
  6. Banovetz, J.T.; Manimaran, S.; Schelske, B.T.; and Anand, R.K. Parallel Dielectrophoretic Capture, Isolation, and Electrical Lysis of Individual Breast Cancer Cells to Assess Variability in Enzymatic Analysis. Anal. Chem. 2023. DOI: 10.1021/acs.analchem.3c00078
  7. Chen, H.; Osman, S.Y.; Moose, D.L.; Vanneste, M.; Anderson, J.L.; Henry, M.D.; and Anand, R.K. Quantification of capture efficiency, purity, and single-cell isolation in the recovery of circulating melanoma cells from peripheral blood by dielectrophoresis. Lab Chip. 2023. DOI: 10.1039/D2LC01113A               
  8. Rahn, K.L.; Peramune, U.; Zhang, T.; and Anand, R.K. Label-Free Electrochemical Methods for Disease Detection. Annu. Rev. Anal. Chem. 2023, 16:1.
  9. Berzina, B.; Peramune, U.; Kim, S.; Saurabh, K.; Claus, E.; Strait, M.; Ganapathysubramanian, B.; and Anand, R.K. ACS Sensors. DOI: 10.1021/acssensors.2c02480
  10. Kim, S.;  Khanwale, M. A.;  Anand, R. K.; Ganapathysubramanian, B., Computational framework for resolving boundary layers in electrochemical systems using weak imposition of Dirichlet boundary conditions. Finite Elements in Analysis and Design. 2022, 205, 103749.
  11. Chen, H.; Anderson, J. L.; Anand, R.K., Electropolymerization of Pyrrole-Based Ionic Liquids on Selected Wireless Bipolar Electrodes. ACS Appl. Mater. Interfaces. 2022, 14, 16, 18087–18096.Figure 1
  12. Krishnamurthy, A.; Anand, R. K., Recent advances in microscale extraction driven by ion concentration polarization. TrAC Trends in Analytical Chemistry. 2022, 148, 116537. Image 1
  13. Berzina, B.; Kim, S.; Peramune, U.; Saurabh, K.; Ganapathysubramanian, B.; Anand, R. K., Out-of-plane faradaic ion concentration polarization: stable focusing of charged analytes at a three-dimensional porous electrode. Lab Chip. 2022, 22, 573-583.Graphical abstract: Out-of-plane faradaic ion concentration polarization: stable focusing of charged analytes at a three-dimensional porous electrode
  14. Borchers, J. S.; Campbell, C. R.; Van Scoy, S. B.; Clark, M. J.; Anand, R. K., Redox cycling at an array of interdigitated bipolar electrodes for enhanced sensitivity in biosensing. ChemElectroChem. 2021 
  15. Rahn, K. L.; Anand, R. K., Recent Advancements in Bipolar Electrochemical Methods of Analysis. Anal. Chem. 2020,  93,  1,  103-123.
  16. Berzina, B.; Anand, R. K., Tutorial Review: Enrichment and Separation of Neutral and Charged Species by Ion Concentration Polarization Focusing. Anal. Chim. Acta. 2020, 1128, 149.
  17. Rahn, K. L.; Rhoades, T. D.; Anand, R. K., Alternating current voltammetry at a bipolar electrode with smartphone luminescence imaging for point-of-need sensing. ChemElectroChem. 2019, 7,  1172.
  18. Kim, S.; Ganapathysubramanian, B.; Anand, R. K., Concentration enrichment, separation, and cation exchange in nanoliter-scale water-in-oil droplets. J. Am. Chem. Soc. 2020, 142, 3196.
  19. Varona, M.; Eitzmann, D. R.; Pagariya, D.; Anand, R. K.; Anderson, J. L., Solid-Phase Microextraction Enables Isolation of BRAF V600E circulating tumor DNA from human plasma for detection with a molecular beacon loop-mediated isothermal amplification assay. Anal. Chem. 2020, 92, 3346.
  20. Rahn, K.; Anand, R. K., Interfacing electronic and genetic circuits. Nat. Chem. 2019, 12, 14.
  21. Anand, R. K.; Baker, L. A.; Sun, L.; Zamborini, F. P.; Zhan, W., A tribute to Richard M. Crooks on the occasion of his 65th birthday. ChemElectroChem 2019, 7, 1062.
  22. Berzina, B.; Anand, R. K., Continuous micellar electrokinetic focusing by ion concentration polarization. Lab on a Chip 2019, 19, 2233.
  23. Banovetz, J. T.; Li, M.; Pagariya, D.; Kim, S.; Ganapathysubramanian, B.; Anand, R. K., Defining cell cluster size by dielectrophoretic capture at an array of wireless electrodes of several distinct lengths. Micromachines 2019, 10, 271. (Invited Article)
  24. Borchers, J. S.; Riusech, O.; Rasmussen, E.; Anand, R. K., Visual voltammogram at an array of closed bipolar electrodes in a ladder configuration. J. Anal. Test. 2019, 3, 150. (Invited Article)
  25. Robole, Z.; Rahn, K.; Lampkin, B. J.; Anand, R. K.; Vanveller, B., Electronic effects and the redox potentials of catechol-boronate complexes. J. Org. Chem. 2019, 84, 2346.
  26. Li, M.; Anand, R. K., Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis. Chem. Sci. 2019, 10, 1506.
  27. Qin, Y.; Wu, L.; Schneider, T.; Yen, G.; Wang, J.; Xu, S.; Li, M.; Paguirigan, A. L.; Smith, J. L.; Radich, J. P.; Anand, R. K.*; Chiu, D. T.* Self-digitization dielectrophoretic (SD-DEP) chip for high efficiency single-cell capture, on-demand compartmentalization, and downstream nucleic-acid analysis. Angew. Chem. Int. Ed. 2018 DOI: 10.1002/anie.201807314.
  28. Berzina, B.; Anand, R. K., An electrokinetic separation route to source dialysate from excess fluid in blood. Anal. Chem. 2018, 90, 3720.
  29. Li, M.; Anand, R. K., Cellular dielectrophoresis coupled with single-cell analysis. Anal. Bioanal. Chem. 2018, 410, 2499. (Invited Review) Full text
  30. Li, M.; Anand, R. K., High-throughput selective capture of single circulating tumor cells by dielectrophoresis at a wireless electrode array. J. Am. Chem. Soc. 2017, 139, 8950.
  31. Li, M.; Anand, R. K., Recent advancements in ion concentration polarization. Analyst 2016, 141, 3496.

Prior to Iowa State University

  1. Johnson, E. S.; Anand, R. K.; Chiu, D. T., Improved detection by ensemble-decision aliquot ranking of circulating tumor cells with low numbers of a targeted surface antigen. Anal. Chem. 2015, 87, 9389.
  2. Anand, R. K.; Johnson, E. S.; Chiu, D. T., Negative dielectrophoretic capture and repulsion of single cells at a bipolar electrode: the impact of faradaic ion enrichment and depletion. J. Am. Chem. Soc. 2015, 137, 776.
  3. Liu, D.; Hakimi, B.; Volny, M.; Rolfs, J.; Anand, R. K.; Turecek, F.; Chiu, D. T., Modulating patterns of two-phase flow with electric fields. Biomicrofluidics 2014, 8, 044106.
  4. Zhao, M.; Nelson, W. C.; Wei, B.; Schiro, P. G.; Hakimi, B. M.; Johnson, E. S.; Anand, R. K.; Gyurkey, G. S.; White, L. M.; Whiting, S. H.; Coveler, A. L.; Chiu, D. T., New generation of ensemble-decision aliquot ranking based on simplified microfluidic components for large-capacity trapping of circulating tumor cells. Anal. Chem. 2013, 85, 9671.
  5. Knust, K. N.; Hlushkou, D.; Anand, R. K.; Tallarek, U.; Crooks, R. M., Electrochemically mediated seawater desalination. Angew. Chem. Int. Ed. Engl. 2013, 52, 8107.
  6. Knust, K. N.; Sheridan, E.; Anand, R. K.; Crooks, R. M., Dual-channel bipolar electrode focusing: simultaneous separation and enrichment of both anions and cations. Lab Chip. 2012, 12, 4107.
  7. Anand, R. K.; Chiu, D. T., Analytical tools for characterizing heterogeneity in organelle content. Curr. Opin. Chem. Biol. 2012, 16, 391.
  8. Sheridan, E.; Hlushkou, D.; Anand, R. K.; Laws, D. R.; Tallarek, U.; Crooks, R. M., Label-free electrochemical monitoring of concentration enrichment during bipolar electrode focusing. Anal. Chem. 2011, 83, 6746.
  9. Anand, R. K.; Sheridan, E.; Knust, K.; Crooks, R. M., Bipolar electrode focusing: faradaic ion concentration polarization. Anal. Chem. 2011, 83, 2351.
  10. Dumitrescu, I.; Anand, R. K.; Fosdick, S. E.; Crooks, R. M., Pressure-driven bipolar electrochemistry. J. Am. Chem. Soc. 2011, 133, 4687.
  11. Anand, R. K.; Sheridan, E.; Hlushkou, D.; Tallarek, U.; Crooks, R. M., Bipolar electrode focusing: tuning the electric field gradient. Lab Chip 2011, 11, 518.
  12. Mavré, F.; Anand, R. K.; Laws, D. R.; Chow, K-F.; Chang, B. Y.; Crooks, J. A.; Crooks, R. M. Bipolar electrodes: a useful tool for concentration, separation, and detection of analytes in microelectrochemical systems. Anal. Chem. 2010, 82, 8766.
  13. Perdue, R. K.; Laws, D. R.; Hlushkou, D.; Tallarek, U.; Crooks, R. M., Bipolar electrode focusing: the effect of current and electric field on concentration enrichment. Anal. Chem. 2009, 81, 10149.
  14. Laws, D. R.; Hlushkou, D.; Perdue, R. K.; Tallarek U.; Crooks, R. M., Bipolar electrode focusing: simultaneous concentration enrichment and separation in a microfluidic channel containing a bipolar electrode. Anal. Chem. 2009, 81, 8923.
  15. Hlushkou, D.; Perdue, R. K.; Dhopeshwarkar, R.; Crooks, R. M.; Tallarek, U. Electric field gradient focusing in microchannels with embedded bipolar electrode. Lab Chip 2009, 9, 1903.
  16. Liu, D.; Perdue, R. K.; Sun, L.; Crooks, R. M. Immobilization of DNA onto poly(dimethylsiloxane) surfaces and application to a microelectrochemical enzyme-amplified DNA hydbridization assay. Langmuir 2004, 20, 5905.
  17. Kijak, A. M.; Perdue, R. K.; Cox, J. A. Modification of electrodes with nanostructured films containing dirhodium-substituted polyoxometalates. J. Solid State Electrochem. 2004, 8, 376.